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CarboPac PA200碳水化合物色譜柱

更新時(shí)間:2019-11-12

簡(jiǎn)要描述:

CarboPac PA200碳水化合物色譜柱
對(duì)于高分辨率測(cè)繪和糖蛋白釋放的寡糖的分析

CarboPac PA200碳水化合物色譜柱

對(duì)于高分辨率測(cè)繪和糖蛋白釋放的寡糖的分析,使用的Thermo Scientific戴安CARBOPACPA200分析及保護(hù)柱提供的敏感性和特異性檢測(cè)。脈沖安培檢測(cè)(PAD)相結(jié)合,此陰離子交換柱提供無(wú)需使用衍生快速,重復(fù)分離。疏水性聚合物,薄殼型陰離子交換樹脂填充材料是在pH值0-14的范圍內(nèi)保持穩(wěn)定。這種*的pH值穩(wěn)定性允許使用有利于碳水化合物的陽(yáng)極氧化的金電極洗脫液組成的。

 

CarboPac PA200碳水化合物色譜柱

訂貨信息:

Dionex CarboPac PA200 碳水化合物分析柱

分析柱
CarboPac PA200 Analytical Column (3 x 250 mm)062896
保護(hù)柱
CarboPac PA200 Guard Column (3 x 50 mm)062895

 

 

Fast, pH-Stable Separation of Oligosaccharides

A variety of HPLC approaches are proposed for characterizing oligosaccharides, but many prove inadequate for separating complex mixtures and are limited by a lack of specificity and high limits of detection. Dionex has developed an improved HPLC technique for carbohydrate analysis based on anion-exchange chromatography coupled with pulsed amperometric detection. This technique provides high-resolution separations with sensitive and specific detection, without derivatization.

  • Sensitive, direct detection with no derivatization, using pulsed amperometric detection

  • Very high-efficiency separations

  • Separations based on size, charge, degree of branching, and linkage isomerism

  • Low flow rate saves on eluent consumption

  • Does not require derivatization

CarboPac? PA200 columns are packed with a hydrophobic, polymeric, pellicular anion-exchange resin stable over the range of pH 0–14. The unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.

 

 

CarboPac PA200 Column for High-Resolution Oligosaccharide Separations Specifications
DimensionsCarboPac? PA200 Analytical Column:
3 × 250 mm
CarboPac PA200 Guard Column:
3 × 50 mm
Maximum operating pressure4000 psi (27.9 MPa)
Temperature range6–60 °C  
Recommended operating temperature: controlled ambient
Typical operating conditions2700 psi at 0.5 mL/min (analytical and guard columns)
Recommended flow rate: 0.5 mL/min
Mobile phase compatibilitypH 0–14; 100% compatible with common organic solvents. 
Ionic form eluents: sodium acetate and sodium hydroxide only.
Resin composition5.5 μm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with 43 nm MicroBead? quaternary amine functionalized latex (6% crosslinked)
Anion-exchange capacityApproximately 35 μeq/column (3 × 250 mm analytical column)
Column constructionPEEK? with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic

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